What You Need to Know About scFv/Fa
Fragment antigen-binding (Fab fragment) is a region on an antibody that binds to antigens. It is composed of one constant and one variable domain of each of the heavy and the light chain. Single-chain variable fragment (scFv) is not actually a fragment of an antibody, but instead is a fusion protein of the variable regions of the heavy (VH) and light chains (VL) of immunoglobulins, connected with a short linker peptide of ten to about 25 amino acids.
Fab fragments differ from scFv's as they contain both variable domains and constant regions, thus are more complex in construction and ultimate expression. Creative Biolabs is an expert with many years of experiences in antibody production. It has established a solid platform for scFv and Fab constuction. The same heavy and light variable chains used for scFv construction can also be used in the construction of Fab.
There are three routes to have a scFv or Fab target a specific antigen in Creative Biolabs. The first approach is to generate a mouse hybridoma clone, then convert full IgG [or IgM] into a scFv or Fab; the second one is to create an immunized phage display scFv or Fab mouse library, then use the antigen to screen the library; the third method is to use the antigen to screen a premade scFv or Fab antibody phage display library [usually a human one] to get scFv or Fab clones directly. We are also able to convert chicken IgY into scFv or Fab fragments.