Wednesday, January 14, 2015

The Construction of scFv/Fab


What You Need to Know About scFv/Fa
 
Fragment antigen-binding (Fab fragment) is a region on an antibody that binds to antigens. It is composed of one constant and one variable domain of each of the heavy and the light chain. Single-chain variable fragment (scFv) is not actually a fragment of an antibody, but instead is a fusion protein of the variable regions of the heavy (VH) and light chains (VL) of immunoglobulins, connected with a short linker peptide of ten to about 25 amino acids.

Fab fragments differ from scFv's as they contain both variable domains and constant regions, thus are more complex in construction and ultimate expression. Creative Biolabs is an expert with many years of experiences in antibody production. It has established a solid platform for scFv and Fab constuction. The same heavy and light variable chains used for scFv construction can also be used in the construction of Fab.

There are three routes to have a scFv or Fab target a specific antigen in Creative Biolabs. The first approach is to generate a mouse hybridoma clone, then convert full IgG [or IgM] into a scFv or Fab; the second one is to create an immunized phage display scFv or Fab mouse library, then use the antigen to screen the library; the third method is to use the antigen to screen a premade scFv or Fab antibody phage display library [usually a human one] to get scFv or Fab clones directly. We are also able to convert chicken IgY into scFv or Fab fragments.

In Creative Biolabs, customers will experience professional and high-qualified scFv/Fab Construction services. To learn more about the procedures, please visit creative-biolabs.com

Monday, January 12, 2015

In Situ Hybridization – An Up-rising Star in the Field of Biology



In situ hybridization (ISH) may not be a new phrase for today’s Biological world. It has not been in this industry for long, but its promising future, without any doubt, is recognized by increasing biologists and institutions.

In situ hybridization (ISH) is a technology being made up of combinations of molecular biology, histochemistry and cytology. Gall from Yale University together with other biologists’ crossing the Xenopus laevis ribosomal genes with its oocyte and mapping the product in 1969, along with Buongiorno Nardell and Amaldi’s mapping the gene of the cell and tissue with isotope-labeled nucleic acid probe in 1970 gave birth to the primitive ISH. As molecular biological technology developed rapidly latter on, especially in the end of 1970s and at the beginning of 1980s when molecular cloning, plasmid and phage DNA were successfully constructed, profound technical foundation were laid to ISH.

In order to support this new technology, many institutions have themselves involved in it. In Creative BioLabs, Customers can experience the most considerable in situ hybridization (ISH) service. They offer a full array of custom in situ hybridization (ISH) service from probe design, tissue acquisition to expert interpretation of gene expression results.

Featured services of Creative Biolabs include template preparation, probe design, labeling and purification, preparation of tissue, pilot hybridization, hybridization and result interpretation. To know more about accurate and reliable In Situ Hybridization procedures, please visit creative-biolabs.com.