Stable, long-term expression of a gene of
interest via stable cell lines
can be either achieved by self-devotion based on vast investment of time and
money, or delivering it to professional commercial biotech companies based on
skilled cell culture scientists and follow-up trouble shooting and letting you
free the time to be focused on more important projects or procedures. If you
are facing the question, which one will you choose?
Whatever the out coming answer is, to know a
more option is always worthy.
Creative
Biolabs has developed a unique series of high-yield
vector system, in which two GC-rich regions flanking the gene expression
cassette makes the promoter and enhancer continuously exposed to
transcriptional machinery by preventing chromatin condensing. This
revolutionary ground-breaking technology allows the available highest yields in
all common mammalian cell protein production host cell lines, such as 293, CHO
and NSO.
With this vector system, antibody yields of
up to 2.0- 5.5 grams/liter [80-120pg/cell/day] have been achieved in chemically
defined, animal component-free medium. The selection strategy has been designed
to identify cell lines that are highly productive and have the ability to grow
well in suspension culture in a state-of-the-art, fed-batch fermentation
process, in which cell density can reach 1.5×107/mL.
Features:
·
Save time and money
·
Eliminate wasteful and time-consuming
errors
·
A whole package of
services, from
Culture
Conditions, Transfection Method, specific Experimental Outline, Protocol for
Batch Culture, Limiting Dilution to Post Transfection etc.
Besides this, Creative Biolabs is also
specialized in Engineered Bacterial construction and large scale fermentation. Take
one of the company’s specific bacterial expression vector— pCD-Bac—for example,
it is a temperature-inducible prokaryotic expression vector that contains a
PRPL promoter, cIts857 gene [temperature-sensitive CI repressor], multiple
cloning sites and two strong transcription terminators. With genetic codons
optimized into that bacterial preference, this vector enables high-yield
protein expression of most proteins in the host bacterial strain Escherichia coli DH5α when its propriety fermentation
protocol is employed using NLF-22 fermentor (Bioengineering).
As I have said in the first place, there is
no need to spend your valuable time and resources on the time-consuming
processes required for stable cell line generation. Just contact the professional outsourcing
company!
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