Accelerate Your Research through Unique Membrane Protein Service

Creative Biolabs is always proud of providing innovatively designed services to propel clients’ researches. Now we practice this principle on membrane protein service again. Unlike conventional over expression techniques with limiting features on several aspects, Creative Biolabs offers different membrane protein expression methods to fulfill the customers' demands.

The alternative expressions of membrane protein make Creative Biolabs outstanding from peer companies:

l   Cell-based Expression (Native Conformation)

Our innovative cell-based expression technologies can best preserve the native conformation of membrane proteins. A wide range of service is provided based on this technology for production, extraction, solubilization, purification, stabilization and crystallization of functional membrane proteins/antigens. 

l   Cell-free Expression (Functional Membrane Protein)

The cell-free expression enables the embedding of membrane proteins in liposomes (bilayer vehicles and planars) directly during the translation step to allow the production of active

"ready-to-use"proteoliposomes. 

l   Virus-like Particles (VLPs)

Expression of viral envelope or capside proteins results in self-assembly of virus-like particles (VLPs). A good possibility to produce membrane proteins in correct functional conformational manner, is to express these proteins in composition of VLPs. VLPs could be used for different approaches, e.g. vaccine development, investigation of receptor functions, screening of antibodies, for immunization and also for purification of different membrane proteins. 

Using these expression systems, we have successfully expressed different types of membrane proteins such as GPCRs, ion channels, porins, viral proteins, transporters, drug receptors, etc. In addition, we provide a centralized area for application of expression and scale-up production of membrane proteins, their purification and characterization.

With our services based on innovative strategies, we keep ourselves being updated all the time to work closely with our consumers and meet their demands. We have been, and will always be committed on accelerating our clients business via better performance .

Interested？Just click www.creative-biolabs.com to learn more!

This is written by Victoria, the editor of Creative Biolabs, a global life science company that is excellent in providing proteinic custom service through its complete system and wealth experienced staff team.

Choosing High-yield Vector System to Generate Stable Cell Lines

Stable, long-term expression of a gene of interest via stable cell lines can be either achieved by self-devotion based on vast investment of time and money, or delivering it to professional commercial biotech companies based on skilled cell culture scientists and follow-up trouble shooting and letting you free the time to be focused on more important projects or procedures. If you are facing the question, which one will you choose? 

Whatever the out coming answer is, to know a more option is always worthy. 

Creative Biolabs has developed a unique series of high-yield vector system, in which two GC-rich regions flanking the gene expression cassette makes the promoter and enhancer continuously exposed to transcriptional machinery by preventing chromatin condensing. This revolutionary ground-breaking technology allows the available highest yields in all common mammalian cell protein production host cell lines, such as 293, CHO and NSO. 

With this vector system, antibody yields of up to 2.0- 5.5 grams/liter [80-120pg/cell/day] have been achieved in chemically defined, animal component-free medium. The selection strategy has been designed to identify cell lines that are highly productive and have the ability to grow well in suspension culture in a state-of-the-art, fed-batch fermentation process, in which cell density can reach 1.5×10⁷/mL.

Features:

·         Stable cell line generation fulfilled by skilled cell culture scientists

·         Save time and money

·         Eliminate wasteful and time-consuming errors

·         A whole package of services, from Culture Conditions, Transfection Method, specific Experimental Outline, Protocol for Batch Culture, Limiting Dilution to Post Transfection etc. 

Besides this, Creative Biolabs is also specialized in Engineered Bacterial construction and large scale fermentation. Take one of the company’s specific bacterial expression vector— pCD-Bac—for example, it is a temperature-inducible prokaryotic expression vector that contains a PRPL promoter, cIts857 gene [temperature-sensitive CI repressor], multiple cloning sites and two strong transcription terminators. With genetic codons optimized into that bacterial preference, this vector enables high-yield protein expression of most proteins in the host bacterial strain Escherichia coli DH5α when its propriety fermentation protocol is employed using NLF-22 fermentor (Bioengineering).

As I have said in the first place, there is no need to spend your valuable time and resources on the time-consuming processes required for stable cell line generation.  Just contact the professional outsourcing company!

Bispecific Antibody by Creative Biolabs

Professionals never cease their interests in studying bi-specific antibody. How much of you know about bispecific antibodies? Bispecific antibodies, BsAbs in short, are bioengineered antibodies that contain two different binding sites within a single molecule, which can specifically bind two different types of antigen.

The most widely used application of bi-specific antibody is in cancer immunotherapy, which creates a therapeutic treatment approach for diseases with specific medical needs.

Compared with traditional antibodies, bispecific antibodies have many advantages. The two arms can bring an antigen-expressing cell in close proximity to a killing cell and thus destroy the tumor cell; the two binding sites can significantly increase affinity or internalization rates of particular antigens on a cell's surface by binding to two different epitopes on an antigen; they usually have a higher cytotoxic potential to bind to antigens with low expression level. Binding tumor cells using a bi-specific antibody-drug conjugate (ADC) also holds great promise for the future development of therapeutics for a variety of diseases.

With the development of antibody engineering, many types of bispecific antibodies have been designed to enable short half-life, immunogenicity and side-effects caused by cytokine liberation. They include tri-functional antibodies, chemically linked Fabs, and various types of bivalent and trivalent single-chain variable fragments (scFvs), fusion proteins mimicking the variable domains of two antibodies. The furthest developed of these newer formats are the bi-specific T-cell engagers (BiTEs) and mAb2's, antibodies engineered to contain an Fcab antigen-binding fragment instead of the Fc constant region. 

As a leader in the field of antibody engineerin, Creative Biolabs has developed CeativeAb^TM antibodies in order to increase the efficacy of current therapeutic antibodies.

Various Types of bispecific antibodies Creative Biolabs offers:

   

u  Tri-functional antibodies and chemically linked Fabs

u  Bivalent,and Bispecific scFvs (tandem scFv, diabodies)

u  Bi-specific T-cell engagers (BiTEs) 

u  scFv-Fab Fusion Proteins

Based on various antibody formats such as IgGs, Fabs and scFvs, Creative Discovery Platform^TM of Creative Biolabs provides a series of bispecific antibodies to satisfy various needs from its customers. It provides construction, including expression and purification as well as recombinant antibody production at scales, ranging from hundreds of micrograms to grams level. 

By clicking Creative Biolabs, you can learn more about bispecific antibody.